gel chromatography

gel chromatography

also called  Gel Filtration,  

      in analytical chemistry, technique for separating chemical substances by exploiting the differences in the rates at which they pass through a bed of a porous, semisolid substance. The method is especially useful for separating enzymes, proteins, peptides, and amino acids from each other and from substances of low molecular weight. The separation of the components of a mixture by gel chromatography is based on the differences in the molecular sizes of the components. Small molecules tend to diffuse into the interior of the porous particles so that their flow is restricted, while large molecules are unable to enter the pores and tend to flow unhindered. Thus, the components of highest molecular weight leave the bed first, followed by successively smaller molecules. The bed materials most extensively used are polyacrylamide and a polymer prepared from dextran and epichlorohydrin. The dry polymers are usually suspended in suitable agents to form a homogeneous, semisolid mixture.

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Universalium. 2010.

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Look at other dictionaries:

  • gel chromatography — n GEL FILTRATION …   Medical dictionary

  • gel chromatography — gelchromatografija statusas T sritis Standartizacija ir metrologija apibrėžtis Chromatografija, kai molekulėms išskirstyti pagal matmenis vartojamas akytas polimero gelis. atitikmenys: angl. gel chromatography; gel filtration; gel permeation… …   Penkiakalbis aiškinamasis metrologijos terminų žodynas

  • gel chromatography — gelchromatografija statusas T sritis chemija apibrėžtis Chromatografija, kai molekulėms išskirstyti pagal matmenis naudojamas akytas polimero gelis. atitikmenys: angl. gel chromatography; gel filtration; gel permeation chromatography rus. гель… …   Chemijos terminų aiškinamasis žodynas

  • gel chromatography — noun see gel filtration …   Useful english dictionary

  • gel filtration — n chromatography in which the material to be fractionated separates primarily according to molecular size as it moves through a column of a gel and is washed with a solvent so that the fractions appear successively at the end of the column called …   Medical dictionary

  • chromatography — chromatographer, n. chromatographic /kreuh mat euh graf ik, kroh meuh teuh /, adj. chromatographically, adv. /kroh meuh tog reuh fee/, n. Chem. the separation of mixtures into their constituents by preferential adsorption by a solid, as a column… …   Universalium

  • gel-permeation chromatography — gelchromatografija statusas T sritis Standartizacija ir metrologija apibrėžtis Chromatografija, kai molekulėms išskirstyti pagal matmenis vartojamas akytas polimero gelis. atitikmenys: angl. gel chromatography; gel filtration; gel permeation… …   Penkiakalbis aiškinamasis metrologijos terminų žodynas

  • gel-permeation chromatography — gelchromatografija statusas T sritis chemija apibrėžtis Chromatografija, kai molekulėms išskirstyti pagal matmenis naudojamas akytas polimero gelis. atitikmenys: angl. gel chromatography; gel filtration; gel permeation chromatography rus. гель… …   Chemijos terminų aiškinamasis žodynas

  • Gel-Permutations-Chromatographie — gelchromatografija statusas T sritis Standartizacija ir metrologija apibrėžtis Chromatografija, kai molekulėms išskirstyti pagal matmenis vartojamas akytas polimero gelis. atitikmenys: angl. gel chromatography; gel filtration; gel permeation… …   Penkiakalbis aiškinamasis metrologijos terminų žodynas

  • gel filtration — gelchromatografija statusas T sritis Standartizacija ir metrologija apibrėžtis Chromatografija, kai molekulėms išskirstyti pagal matmenis vartojamas akytas polimero gelis. atitikmenys: angl. gel chromatography; gel filtration; gel permeation… …   Penkiakalbis aiškinamasis metrologijos terminų žodynas

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