Kohler, Georges J.F.

▪ 1996

      German immunologist (b. April 17, 1946, Munich, Germany—d. March 1, 1995, Freiburg im Breisgau, Germany), shared the 1984 Nobel Prize for Physiology or Medicine for his contribution to the discovery (1975) of a method of producing unlimited amounts of extremely pure proteins known as monoclonal antibodies, each of which could be directed against a specific antigen. These antibodies—and the subsequent developments based on the work done by Köhler and his partner and colaureate, César Milstein—revolutionized the diagnosis and treatment of many immunologic diseases, including some forms of cancer and AIDS. In 1974 Köhler received his doctorate in biology from the University of Freiburg and joined Milstein at the Medical Research Council Laboratory of Molecular Biology, Cambridge, England. Within months they had made their antibody breakthrough. Köhler left to conduct research (1976-84) at the Basel (Switz.) Institute for Immunology, where colaureate Niels K. Jerne had first proposed his Nobel Prize-winning theories on antibodies and the human immune system. Köhler was appointed a director of the Max Planck Institute for Immunobiology in Freiburg in 1985.

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▪ German immunologist

in full  Georges Jean Franz Köhler 

born April 17, 1946, Munich, Ger.

died March 1, 1995, Freiburg im Breisgau

 German immunologist who in 1984, with César Milstein (Milstein, César) and Niels K. Jerne (Jerne, Niels K.), received the Nobel Prize for Physiology or Medicine for his work in developing a technique for producing monoclonal antibodies (monoclonal antibody)—pure, uniform, and highly sensitive protein molecules used in diagnosing and combating a number of diseases (see illustration—>).

      Köhler obtained his doctoral degree in biology (1974) from the University of Freiburg in West Germany. From 1974 to 1976 he worked with Milstein at the Medical Research Council Laboratory of Molecular Biology in Cambridge, England. Together, in 1975, they discovered the technique for which they are known.

      In the body's immune system, cells called lymphocytes secrete various types of antibodies (antibody), whose function is to attach themselves to antigens (antigen) (foreign substances) that have entered the body. The immune system maintains a vast variety of antibodies, with each type able to attach itself to a matching site on the surface of a particular type of antigen (e.g., a particular species or strain of bacteria). To prepare substantial quantities of antibodies, scientists used to inject an antigen into an animal, wait for antibodies to form, draw blood from the animal, and isolate the antibodies. The antibodies obtained by this procedure were almost never pure, because typical antigens possess many recognizable surface sites, each of which leads to formation of a different type of antibody.

      Köhler and Milstein saw that if a way could be found to clone lymphocytes—to cause them to subdivide indefinitely in a culture medium—then the antibody molecules secreted by the resulting population would all be identical. Lymphocytes are short-lived, however, and cannot be cultivated satisfactorily. Köhler and Milstein solved this problem by inducing lymphocytes to fuse with the cells of a myeloma (a type of tumour), which can be made to reproduce indefinitely. The resulting hybrid cells produced a single species of antibody while perpetuating themselves indefinitely.

      The development of monoclonal antibodies revolutionized many diagnostic procedures and led to new therapeutic agents for fighting disease, since monoclonal antibodies can be designed to target specific types of cells or other antigens and can be used to carry drugs to those cells.

      Köhler worked at the Basel Institute for Immunology from 1976 to 1985. In 1985 he was appointed one of three directors of the Max Planck Institute for Immunobiology in Freiburg.

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