Northern blot

Northern blot
Biol., Med. a procedure for studying the activity of a specific gene, as in testing for a hereditary defect: RNA fragments in an extract of a cell population or a tissue are separated by gel electrophoresis, transferred to a filter, and then hybridized with labeled DNA or RNA derived from a laboratory-engineered copy of the gene in order to identify the size and abundance of the RNA expressed by that gene.
[1977; orig. as a facetious counterpart to SOUTHERN BLOT]

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Universalium. 2010.

Look at other dictionaries:

  • Northern Blot — Un transfert d ARN[1] (également appelé northern blot(en) ou buvardage de northern), est une méthode de biologie moléculaire permettant l analyse de l ARN. Elle dérive du Southern blot (appelé ainsi par le biologiste Edwin Southern qui a aussi… …   Wikipédia en Français

  • Northern blot — The northern blot is a technique used in molecular biology research to study gene expression by detection of RNA (or isolated mRNA) in a sample.[1][2] …   Wikipedia

  • Northern blot — En este artículo sobre biología se detectaron los siguientes problemas: Necesita ser wikificado conforme a las convenciones de estilo de Wikipedia. Carece de fuentes o referencias que aparezcan en una fuente acreditada. Po …   Wikipedia Español

  • Northern-Blot — schematischer Aufbau eines Northern Blot Der Northern Blot ist eine molekularbiologische Methode zur Übertragung (Blotten) der in der Gelelektrophorese aufgetrennten RNA auf eine Membran (Diazobenzyloxymethyl (DBM) Papier oder unter bestimmten… …   Deutsch Wikipedia

  • Northern Blot — schematischer Aufbau eines Northern Blot Der Northern Blot ist eine molekularbiologische Methode zur Übertragung (Blotten) der in der Gelelektrophorese aufgetrennten RNA auf eine Membran (Diazobenzyloxymethyl (DBM) Papier oder unter bestimmten… …   Deutsch Wikipedia

  • Northern blot — A technique in molecular biology, used mainly to separate and identify pieces of RNA. Called a Northern blot only because it is similar to a Southern blot (which is named after its inventor, the British biologist M.E. Southern). There is also a… …   Medical dictionary

  • Northern blot — Un transfert d ARN[1] (également appelé northern blot (en) ou buvardage de northern), est une méthode de biologie moléculaire permettant l analyse de l ARN. Elle dérive du Southern blot (appelé ainsi par le biologiste Edwin Southern qui a aussi… …   Wikipédia en Français

  • northern blot — noun Usage: usually capitalized N Etymology: after Southern blot (herein) : a blot consisting of a sheet of cellulose nitrate or nylon that contains spots of RNA for identification by a suitable molecular probe compare southern blot herein… …   Useful english dictionary

  • Northern blot — An electroblotting method in which RNA is transferred to a filter and detected by hybridization to32 P labelled RNA or DNA. See blots …   Dictionary of molecular biology

  • northern blot — A technique analogous to Southern blotting, but involving the transfer of RNA from an agarose gel to a membrane, prior to probing …   Glossary of Biotechnology

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